| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 봄 (04/22 ~ 04/23, 대구 EXCO) |
| 권호 |
16권 1호, p.383 |
| 발표분야 |
생물화공 |
| 제목 |
Cellulosic Ethanol fermentation Using RecombinantSaccharomyces cerevisiae Engineered for Production of minicellulosome |
| 초록 |
Lignocellulosic biofuels have been widely regarded as the only foreseeable alternative to conventional transport fuels.In this study,we report engineering of Saccharomyces cerevisiae for assembly of minicellulosomes by heterologous expression of a recombinant scaffolding protein and a chimeric endoglucanase. In this proposed minicellulosome by using cohesin-dockerin interaction, the recombinant scaffolding protein mini-CbpA containing two cohesin domains of CbpA from Clostridium cellulovorans was expressed. To assemble the minicellulosome, we constructed a chimeric CelE containing the catalytic domain of CelE fused with dockerin domain of C. cellulovorans EngB. The resulting strain was able to ferment amorphous cellulose into ethanol with the aid of β-glucosidase 1. The minicellulosome assembled in vivo is useful for direct ethanol production with synergic effect from cellulosic substrates. The development of a more effective cellulosic ethanol fermentation process is required to bring about a necessary dramatic reduction of production costs. So this strain will lead to more efficient ethanol production. |
| 저자 |
한성옥 |
| 소속 |
고려대 |
| 키워드 |
minicellulosome; ethanol fermentation; Saccharomyces cerevisiae; Clostridium cellulovorans
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| E-Mail |
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| 원문파일 |
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