| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Nano-Complex of Protein/Polysaccharide for Protein Stability in Poly(lactide-co-glycolide) Microspheres |
| 초록 |
An acidic mucopolysaccharide is able to form ionic complexes with positively-charged proteins. In this study, a protein-acidic mucopolysaccharide complex was constructed to nano-sized particles. Zeta potential measurements revealed that acidic polysaccharide -to-protein fraction of greater than 0.1 results in a neutralization of the positive charge on lysozyme (Lys). Based on this preliminary study, we have prepared poly(lactide-co-glycolide) (PLGA) microspheres harboring Lys/acidic polysaccharide complexes via the multi-emulsion method. The profiles of Lys release from the PLGA microspheres evidenced nearly zero-order kinetics, depending on the quantity of acidic polysaccharide. The tissue reaction evidenced by the PLGA microspheres stabilized with acidic polysaccharide showed minimal foreign body reaction and little configuration of immune cells including neutrophils and macrophages, but the reactions of the PLGA microspheres without acidic polysaccharide were characterized by a relatively elevated inflammation. These results show that acidic polysaccharide is a viable candidate for long-acting micro-particular protein delivery. |
| 저자 |
KUN NA |
| 소속 |
Division of Biotechnology |
| 키워드 |
acidic mucopolysaccharide; Ionic complex; Protein Stabilization; Poly(lactide-co-glycolide) Microsphere. |
| E-Mail |
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