| 초록 |
The increasing need for enantiomerically pure chiral compounds has accelerated development of various chemical and physical methods of kinetic resolution and asymmetric synthesis. To this end, ω-transaminase(ω-TA) can be employed for kinetic resolution or asymmetric synthesis of chiral amine. Simultaneous production of L-homoalanine and (R)-α-methylbenzylamine from racemic α-methylbenzylamine and L-threonine was achieved using an ω-TA and threonine deaminase (TD) in a coupled reaction. To this purpose, (S)-selective ω-TA from Ochrobactrum anthropi and TD(ilvA) from Escherichia coli were cloned into a pRSF duet vector and functionally expressed in Escherichia coli BL21 cells. In whole cell reaction, TD converts L-threonine 2-oxobutyrate which is used as an amino acceptor for ω-TA. As a result, optically pure (R)-α-methylbenzylamine and L-homoalanine could be obtained and the method is applicable to kinetic resolution of various chiral amines. |
