| 초록 |
Microalgae have garnered considerable interest as CO2 reduction platforms as well as next-generation energy and food sources. Despite the potential, actual utilization of microalgae as a CO2 reduction technology through large-scale cultivation is limited mainly due to the biological platforms' low tolerance to environmental fluctuations. Especially, microalgae's inherent low tolerance to high CO2 is largely responsible for the limitation. To solve the problem, we tried to maximize microalgal ability to maintain pH homeostasis by heterologous expression of proton (H+) pumping plasma membrane ATPase since it is widely accepted that pH tolerance governs CO2 tolerance of microalgae. As a result, pH tolerance of a green microalga, Chlamydomonas reinhardtii was markedly increased at low pH regime. Thanks to the heterologous protein expression, CO2 tolerance was simultaneously increased and thus CO2 fixation under a high CO2 level (ca. 15%) was almost doubled. |
