| 초록 |
In this study, a novel split mixotrophic cultivation strategy(SMCS) developed as better mixotrophy via offering mutual benefits through gas exchange at both headspaces while splitting autotrophic and heterotrophic modes. To quantify synergistic growth effects in combined-autotrophy and combined-heterotrophy(CA&CH) of SMCS, gross O2 evolution, DIC and DO concentrations were compared with those of autotrophic, heterotrophic and mixotrophic condition. Average 12–14% and 26–32% increase in DIC and DO concentrations were determined respectively in CA and CH than A, H and M. Biomass yield in CA+CH was increased approx.1.5 folds higher than yields of A + H and M regimes. These results show SMCS as better cultivation strategy than the M by increased biomass and lipid yields. Challenges associated with organic carbon can be solved by SMCS viz. chlorophyll loss, organic carbon uptake inhibition. |
