| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
19권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
On-bead cell-free protein synthesis of parasite antigenic candidates for high-throughput expression and screening |
| 초록 |
Screening of suitable antigen is an important step in the development of diagnostic and therapeutic tools in the medical field. This study describes high-throughput expression screening of parasite based on the techniques of cell-free protein synthesis. The libraries of Plasmodium vivax and Clonorchis sinensis genes were PCR-amplified and immobilized on streptavidin agarose beads. Through dual functionalization of the agarose beads to capture 6xhistidine-tagged proteins as well as the biotinylated PCR products, when expressed in a reaction mixture for cell-free synthesis, template DNA and the expressed protein were in situ immobilized on the same beads. This approach of in situ expression and isolation enables streamlined recovery and analysis of cell-free synthesized proteins and also allows facile identification of the genes coding antigenic proteins through direct PCR of the microbead-bound DNA. |
| 저자 |
김동명1, 주정원2, 김유정2, 신현일2, 이승원1, Devi Kasi1, Christy Catherine1, 이경호1
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| 소속 |
1충남대, 2질병관리본부 |
| 키워드 |
Cell-free protein synthesis; Parasite antigen screening; Solid phase protein synthesis
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| E-Mail |
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