| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Screening of cell-penetrating antibodies using a cell-free protein synthesis system |
| 초록 |
With its unique open reaction format, cell-free protein synthesis provides a versatile platform for production and screening of heterologus recombinant proteins. In this study, we used the cell-free protein synthesis system for expression screening of cell-penetrating scFv antibodies from a designed mutant library. By streamlining cell-free expression of the library with a cell-based split GFP complementation assay, we could examine the cell-penetration efficiency and stability of individual mutant scFvs in a fast and parallel manner. Cell-free protein synthesis system enables direct access and control over translational conditions, which was proven to be advantageous in high-throughput screening of antibodies. |
| 저자 |
Christy1, Devi Kasi1, 이경호1, 박유진1, 박성욱2, 김용성2, 김동명1
|
| 소속 |
1충남대, 2아주대 |
| 키워드 |
Cell-free protein synthesis; cell-penetrating scFv; GFP complementation
|
| E-Mail |
|
