| 초록 |
Syngas fermentation is a promising technology to produce valuable chemicals, and fuels. However, there are several issues, yet to address for scale-up of the technology. These include: low mass transfer of gaseous substrate (CO, H2, and CH4) and low cell, and product concentrations. In this study, we proposed a new and innovative reaction system to achieve high cell and product formations called “Simultaneous Cell and Gas Recycled (SCGR) continuous reaction” in syngas fermentation. A high gas-liquid mass transfer system assisted with continuous recycling of cell is employed to ferment CO using Eubacterium limosum (Elm) KIST612. Elm strain uses CO as sole carbon and energy source and produces acetate, and butyrate. During the SCGR continuous reactor operation, Elm was cultivated on carbonate-buffered basal medium (CBBM), while gaseous mixture (CO:CO2:4:1) was spargerd in BCR at a constant flow rate of 5 ml/min. The reactor was operated under fed-batch mode before being shifted to SCGR mode. SCGR mode finally resulted in high cell concentration (5.0 g/L) and acetate production (8.1 g/L), respectively. |
