초록 |
In this work, Coptidis Rhizoma is a traditional medicine used for antipyretic, blood pressure, antiinflammatory et al. From the result, the 100% water, 100% Ethanol and 50% Ethanol each extraction solvent that applied dipping and extraction time 4hr, at 25 ºC. After pretreatment. The free radical scavengers in the Coptidis Rhizoma were screened, and identified by off-line ABTS, DPPH-assay and on-line screening HPLC-ABTS assay coupled. The confirms the feasibility of assessing bioactivity of specific phytochemicals using the on-line screening HPLC-ABTS assay method. |