| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
The comparison of sponge and PLGA scaffold impregnated DBP on growth of Human Intervertebral Disc Cells |
| 초록 |
We fabricated DBP sponge and PLGA scaffolds impregnated demineralized bone particle(DBP)(DBP/PLGA) and investigated proper condition to proliferation and phenotype maintenance of intervertebral disc(IVD) cells by comparison between DBP/PLGA scaffold and DBP sponge. Human IVD cells were seeded in scaffolds of two types. Cell viability and proliferation according to scaffolds were analyzed by MTT assay. RT-PCR was assessed to measure mRNA expression of aggrecan and type II collagen of human IVD cells. In MTT assay results, cell viability in scaffolds impregnated DBP/PLGA scaffold was higher than DBP sponge. We could observe that cells were attached and proliferated and that disc cell mRNA expressed better in DBP/PLGA scaffold than DBP sponge. We concluded that the using of DBP/PLGA in terms of scaffold fabrication for bio-disc with human IVD cells is helpful growth of disc cells maintenance of phenotypes. This research was supported by MHWFA(0405-BO01-0204-0006) and WCU(R31-20029). |
| 저자 |
이선경, 김순희, 홍희경, 김용기, 이동원, 이종문, 강길선
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| 소속 |
전북대 |
| 키워드 |
DBP/PLGA scaffold; DBP sponge; Human disc cell
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| E-Mail |
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