| 학회 |
한국화학공학회 |
| 학술대회 |
2010년 가을 (10/21 ~ 10/22, 대전컨벤션센터) |
| 권호 |
16권 2호, p.1510 |
| 발표분야 |
생물화공 |
| 제목 |
Development of DNA ligase-based system for accurate discrimination of C/T type SNP using modified DNA probe |
| 초록 |
SNPs (single nucleotide polymorphisms) are one-base DNA sequence variations that are responsible for various inherited diseases. DNA ligase-based mismatch detection methods have been proposed as useful strategies for analyzing SNPs, but the methods showed unavoidable limitation in cytosine/thymine(C/T)-type SNP analyses. Here, we employed DNA oligomers containing chemically modified nucleobases at the end as new ligation fragments for DNA-ligase-based SNP analysis. The use of oxanine adjacent to guanine in the ligation junction led to successful ligation for G:C and no ligation for G:T. That is, this method provides clear discrimination of guanine:thymine (G:T) mismatch from guanine:cytosine (G:C). This ligation method using an oxanine-containing fragmentwill be useful for developing accurate C/T-type SNP analysis system. |
| 저자 |
백승필1, 장의경1, 여기백1, Keisuke Makino2
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| 소속 |
1고려대, 2Kyoto Univ. |
| 키워드 |
DNA ligase; SNP analysis; modified nucleobase
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| E-Mail |
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| 원문파일 |
초록 보기 |
