초록 |
We have been study on the production of ethanol by Zymomonas mobilis(KCTC, 1534) fermentation with chemically saccharified methyl cellulose(MC). For the purpose of remove the remain radicals, sonication was performed before the fermentation. Z. mobilis fermented with sonicated materials as a glucose substrate in the RM modified medium for 48hr at 33℃. The maximum yield of ethanol is 0.02 g/g from sonicated substrate whereas is 0.0017 g/g from untreated substrate, 91.5% increased. The radical removal efficiency was analyzed by of 2,2-diphenyl-1-picryl-hydrazyl(DPPH) UV absorption method. The saccharide with chemically depolymerized methyl cellulose was determined by the 3,5-dinitrosalicylic acid(DNS) method. As determined by DNS reducing sugar concentration was 13g/L at MC 50 wt% treated with H2O2, Fe ion at 100℃ for 6hr. |