| 초록 |
High signal amplification is essential for ultrasensitive detection of biomolecules. In recent years, we have developed new redox cycling schemes combined with enzymatic amplification that allow high signal amplification in a simple format along with low background levels. To minimize unwanted side reactions, a new concept that outer-sphere-reaction-philic species can react slowly with inner-sphere-reaction-philic species was applied. In this presentation, the signal amplification based on (i) electrochemical-chemical (EC) redox cycling using a reducing agent, (ii) electrochemical-chemical-chemical (ECC) redox cycling using a reducing agent and an oxidizing agent, and (iii) electrochemical-enzymatic (EN) redox cycling using a redox enzyme will be introduced. Moreover, our efforts for practically useful biosensors (lateral-flow immunosensors and washing-free immunosensors) will be presented. |
