| 초록 |
Over the last few decades, significant efforts have been involved to develop rapid, accurate, reproducible and portable diagnostic technologies. Recently, a Field-effect transistor (FET) has been used as diagnostic tools. It is gated by changes of charge carrier density in the channel induced by the binding of target molecules, leading to electrical signal. Herein, we introduced a high performance FET-typed sensor platform with G-protein- coupled receptor (GPCR). It could provide the opportunity a variety of sensor such as disease diagnosis, drug discovery, and food-spoilage in the future. In this study, we proposed an approach for studying receptor agonism and antagonism by combining the roles of FETs and GPCRs in a dopamine receptor D1 (DRD1)-conjugated FET system, which is a suitable substitute for conventional cell-based receptor assays. The real-time responses from the DRD1-nanohybrid FET were highly sensitive and selective for dopamine agonists/antagonists, and their maximal response levels were clearly different depending on their DRD1 affinities. |
