| 초록 |
Gene carrier nanoparticles with minimal toxicity and high transfection efficiency were fabricated from biodegradable polymer (L-tyrosine polyurethane, LTU). In order to evaluate cellular uptake and transfection studies, we prepared fluorescently labeled bovine serum albumin (FITC-BSA) to investigate cellular uptake and pDNA-linear polyethylenimine (LPEI) complex to investigate the transfection efficiency in LX2, HepG2, MCF7 cells. And the second study was progressed with biodegradable polyfumarateurethane (PFU) for use as a bupivacaine delivery vehicle, synthesized using di-(2-hydroxypropyl fumarate) (DHPF), polyethylene glycol (PEG) and 1,6-hexamethylene diisocyanate (HMDI), was designed to be degradable through the hydrolysis and enzymatic degradation of the ester bonds in its polymer backbone. Using double emulsion techniques, LTU and PFU nanoparticles were fabricated encapsulating the drug or gene, to avoid the immune system their surface was modified with PEG. |
