| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
19권 2호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Conversion of lysine into cadaverine by whole cell biocatalysts using Escherichia coli ldcC |
| 초록 |
1,5-diaminopentane(cadaverine) is a valuable chemical because of its wide industrial applications. Since there are not many reports of the direct production using biocatalysts, the enzymatic process using lysine decarboxylases(LDCs) was developed for cadaverine production from renewable material such as lysine. There are two well-known LDCs in Escherichia coli, cadA and ldcC. Among them, cadA is more popular than ldcC because cadA has two times higher biological activity in comparison with ldcC. In this study, lysine was converted into cadaverine by whole cell biocatalysts using E.coli BL21(DE3) expressing E.coli ldcC. In order to overcome the low expression level and the low conversion rate of ldcC, the effects of IPTG concentration and conversion temperature and pH were studied to increase the expression of ldcC and the conversion rate of lysine into cadaverine. Higher expression level of ldcC was observed, and 95% of lysine was consumed for conversion into cadaverine. |
| 저자 |
박경문1, 양영헌2, 송헌석2, 이주희2, 김정호2, 김현중2, 신지현1
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| 소속 |
1홍익대, 2건국대 |
| 키워드 |
lysine; cadaverine; whole cell conversion
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| E-Mail |
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