| 학회 | 한국재료학회 |
| 학술대회 | 2016년 가을 (11/16 ~ 11/18, 경주 현대호텔) |
| 권호 | 22권 2호 |
| 발표분야 | B. 나노화학/바이오 재료 분과 |
| 제목 | Fabrication of a surface-modified nucleic acid capturing filter for direct DNA extraction and gene expression analysis |
| 초록 | We have developed a surface-modified nucleic acid capturing filter (SNAF) via initiated chemical vapor deposition (iCVD) process by using a poly(dimethylaminomethylstyrene) (PDMAMS) which is capable of in situ nucleic acid extraction and direct application to the gene expression analysis. The SNAF has various advantages of simple fabrication process, superfluous toxic solvents, uniform surface coverage, and unnecessary of after-synthesis purification. Furthermore, the SNAF can be directly captured nucleic acids without inhibitory chemicals to nucleic acid amplification and additional instrumentations which makes possible to overcome the limitations of conventional extraction method. The conformal coating of PDMAMS on the substrate was observed using SEM by confirming the surface morphology and cross section of the SNAF. The XPS results indicate that the PDMAMS coating was successfully performed as well. The nucleic acid capturing capability of the SNAF was demonstrated by using a confocal microscope, Raman spectroscopy, and zeta potential analysis. To evaluate the high-performance of the SNAF, DNA from Escherichia Coli O157:H7 (E. coli O157:H7) was recovered by immersing the filter into the cell lysate with maximum capturing capability of 37 ng/mm2 which was corresponding to ~6.1 × 106 copies/mm2 of nucleic acids in case of E. coli O157:H7. The recovered DNA on the SNAF could directly be amplified via PCR, and subsequently detected by fluorescence with limit of detection (LOD) with 1.67 × 103 cells. Foodborne pathogen can be detected on artificially infected food sample using the SNAF, demonstrating its potential as essential platform for next-generation point-of-care technology. |
| 저자 | 김용태1, 유재범2, 이경균1, 최윤호2, 김지현1, 최성균1, 김경훈1, 이태재1, 임성갑2, 이석재1 |
| 소속 | 1나노종합기술원, 2한국과학기술원 |
| 키워드 | initiated chemical vapor deposition (iCVD); nucleic acid extraction; cationic polymer; pathogen detection |
