| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 |
19권 1호 |
| 발표분야 |
생물공학_포스터 |
| 제목 |
Cloning-independent engineering of elastin-like polypeptides using unnatural amino acids. |
| 초록 |
Based on the central dogma of protein synthesis, traditional methods for protein engineering require that altering protein structure and function must be accompanied by changing the nucleotide sequence of the genes encoding the protein. However, the preparation of a template gene for each individual protein requires a great deal of time and effort, thereby limiting the throughput and scope of studying engineered proteins. In this study, we describe translation-level engineering of proteins using cell-free protein synthesis. Taking advantage of the promiscuity of aminoacyl tRNA synthetases in accepting structurally similar amino acid analogues, unnatural amino acids were introduced into elastin-like polypeptides in place of the corresponding cognate amino acids. Through the incorporation of various analogues and starting from the same gene, the phase transition temperatures of elastin-like polypeptides became tunable. Our results demonstrate the usefulness of cell-free protein synthesis for protein engineering using unnatural amino acids without the need for cloning. |
| 저자 |
Christy1, 오수진1, 민승의1, 원종인2, 윤현동3, 김동명1
|
| 소속 |
1충남대, 2홍익대, 3건국대 |
| 키워드 |
Cell-free protein synthesis; ELP; Unnatural amino acid
|
| E-Mail |
|
