화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU))
권호 22권 2호
발표분야 생물공학_포스터
제목 Biosynthesis β-amino acid using ELP-fused ω-transaminase.
초록 Optically pure amines, β- amino acids and γ-amino acids are the valuable precursors to produce biologically active compounds. The ω-TA are the class of enzymes which are widely used to produce such compounds. In this work (S)-ω-transaminase was fused with Elastin-like polypeptides (ELPs) through the cloning process and expressed in E. coli cells. The characterization of this fusion complex was performed with respect to thermostability and effect of DMSO was checked. Where in case of ω-TA-ELP-V60, major difference in the Tt was observed, wherein a Tt of 38 and 70 οC was observed at the increasing concentration of DMSO from 5 to 25% (v/v). Interestingly, these fusion proteins the activity was preserved even after the aggregation of fusion complex at transition temperature (Tt). The substrate specificity and product inhibition analysis showed that ω-TA-ELPs had comparable results as that of wild type ω-TA.
저자 전현우, 윤상한, Sharad sarak, Mahesh Patil, 유소정, 윤형돈
소속 건국대
키워드 (S)-ω-transaminase; Elastin-like polypeptides; Chiral amines; Bio-catalysis; Thermostability.
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