| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 봄 (04/09 ~ 04/10, 대전컨벤션센터) |
| 권호 |
34권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Tissue engineering for cartilage regeneration using a Hyaluronic acid/ PLGA scaffold : in vivo test |
| 초록 |
PLGA and hyaluronic acid(HA) has been widely used as biocompatible scaffolds materials to regenerate tissue. In this study we prepared HA/PLGA scaffolds by emulsion freeze-drying method. Chondrocytes were isolated from the articular cartilage of rabbit and seeded on the HA-PLGA scaffolds. After 2weeks of cell seeding, we implanted HA/PLGA scaffolds on the back of nude mice. Morphological observation, histology, biological assay for collagen and sGAG, and PCR were performed at each time point 1, 2, 3 and 6 weeks. Specific mRNA, B-actin and collagen type II, for chondrocyte and cell distribution expressed highly in HA/PLGA scaffold while dedifferentiation marker. Although collagen, sGAG, collagen type II and aggrecan were greater than HA-PLGA scaffolds. Therefore, this study suggests that HA/PLGA scaffold may serve as a potential cell delivery vehicle and a structural basis for in vivo tissue engineered articular cartilage.This work was supported by SCRC(SC4110). |
| 저자 |
김혜린, 정수현, 김수진, 김순희, 유일수, 이동원, 이종문, 강길선
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| 소속 |
전북대 |
| 키워드 |
scaffold; Hyaluronic acid; PLGA; chondrocyte
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| E-Mail |
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