| 초록 |
In this study, we have suggested a new material, pH-sensitive polymer, as a new material for enzyme-free stem cell detachment. We synthesized biocompatible polymer which can undergo fast pH-sensitive degradation without causing cytotoxicity. The polymer was coated on the surface of tissue culture plates for stem cell culture and low pH solution (pH 6.0 PBS) was applied afterward to detach the cells without using conventional enzyme treatment. The conventional method that requires enzyme such as trypsin-ethylene diamine tetra acetic acid can cause cellular damage during the cell detaching procedure. Human adipose derived stem cells (hADSCs) detached from the polymer coated plates with pH 6.0 polymer showed no evidences related with cellular damage and showed promising angiogenic efficacy in both in vitro and in vivo experiments. |
