| 초록 |
Biodegradable poly(lactide-co-glycolide) (PLGA) scaffolds for tissue regeneration have been widely studied. However, the inflammatory reaction would be arisen by acidic decomposition product from PLGA. Recently, we have carried out to decrease inflammation and improve the biological function through Mg(OH)2, and extracellular matrix (ECM), PLGA/ECM/Mg(OH)2. In this study, we researched to add extracellular vesicle (EV) known to have the ability to regeneration into these scaffolds. The yield of EV isolation was improved through the serial extrusion, loaded EV into the PLGA/ECM/Mg(OH)2 through the simple coating and covalent conjugation, and then confirmed the modification using scanning electron microscopy (SEM) and fluorescence microscopy. The tissue regenerative potential of PLGA/ECM/Mg(OH)2/EV scaffold was investigated in vitro. Finally, the scaffold prepared with the optimized condition will be further utilized in an animal model for tissue regeneration. |
