| 초록 |
Microalgae which is the third generation types of biomass have received much attention as an alternative energy sources to fossil fuel due to their beneficial traits. They require short cultivation time and small land of cultivation. They accumulate a large amount of lipids by consuming carbon dioxide in atmosphere. Also, the use of biodiesels that produced from microalgal biomass is reported to be more effective in reducing exhausted gas. However, microalgal biofuel production cost is much higher than other fuels due to its raw material cost. To reduce it, microalgal strain should be developed to produce a large amount of lipids in their body. In this study, development of high-lipid accumulating Nannochloropsis salina was conducted by using insertional mutagenesis method and fluorescence-activated cell sorting strategy. The obtained mutant showed enhanced growth rate by 50% than wild-type. Fatty acid methyl ester (FAME) content of isolated mutant was increased by 30%. Also, biomass productivity this mutant was improved by 30% and FAME productivity was enhanced by 70%. It is expected that isolated mutant strain can contribute to reduce raw material cost of microalgal biofuels. |
