| 학회 | 한국화학공학회 |
| 학술대회 | 2003년 가을 (10/24 ~ 10/25, 한양대학교) |
| 권호 | 9권 2호, p.2100 |
| 발표분야 | 생물화공 |
| 제목 | A study on the development of interferometric biochip to sense β-galactosidase releasing by recombinant E. coli responding to an environmental endocrine disruptor(bisphenol A) |
| 초록 | A recombinant E.coli ACV1003(recA::lacZ) releasing β-galactosidase by a SOS regulon system, when it is exposed to a DNA-damaging compound, has been used to detect easily endocrine disruptors such as a bisphenol A. Heavily doped p-type porous silicon was fabricated by etching to produce a Fabry-Perot fringe pattern, which caused the change in the refractive index of the medium including β-galactosidase. Surface of porous silicon has been modified and funtionalized with (3-mercaptopropyl)trimethoxysilane, using SAM(self-assembled monolayer) to display considerable stability against detachment of the β-galactosidase molecules, and then environmental endocrine disruptor can be easily determined by the changes in the refractive index of the medium upon enzyme-ligand binding. The change in the effective optical thickness versus β-galactosidase showed a sigmoid increase up to the concentration of 200 unit β-galactosidase ml-1. |
| 저자 | 임성혁, 김수영, 박재숙, 김병우 |
| 소속 | 성균관대 |
| 키워드 | biosensor; Fabry-Perot; porous silicon |
| 원문파일 | 초록 보기 |
