| 초록 |
A multi-specific drug carrier provides synergistic therapeutic avenues by engaging multiple targets that are pathologically associated. Scaffolds like antibody-drug conjugates and bispecific antibodies have demonstrated their potentials in clinical trials. Human serum albumin (HSA) is deemed a promising drug delivery scaffold for its excellent serum stability and structural tolerance to modification. In this study, we engineered HSA as a multi-specific drug carrier. In order to install a modular drug-binding motif, a part of HSA was substituted with BOLT domain, resulting in a novel multi-specific albumin carrier (MAC) capable of co-assembling with a payload tagged by NUT domain. Binding assays revealed that MAC could specifically bind a model protein, GFP-NUT, with a nanomolar affinity, suggesting its potential as a modular drug carrier. We are currently investigating the binding behavior of MAC towards human FcRn which is critical for surviving longer in the systemic circulation. |
