| 초록 |
New metal-enhanced fluorescence (MEF)-based biosensor platform was prepared on the fibrous substrates. First PCL fibers were obtained by electrospinning. Then, PCL fibers were decorated with silver nanoparticles and then, further coated with silica layer. The silica layer acted as a spacer between silver nanoparticles and fluorescence molecules. The MEF was obtained from silver-decorated PCL fibers with silica layer, extent of which could be controlled by the thickness of silica layer. The fibrous structure of Ag@SiO2-PCL had higher protein loading capacity than conventional two-dimensional glass slide due to its large surface area, while the presence of silica-coated silver nanoparticles resulted in higher fluorescence intensity than bare PCL fibers via MEF effect. According to immunobinding assays between fluorescence-labeled anti-IgG and IgG immobilized onto different substrates, Ag@SiO2-PCL had about 42.5 and 2.9 times higher sensitivity than glass slide and bare PCL fibers. |
