| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 |
14권 1호, p.1232 |
| 발표분야 |
재료 |
| 제목 |
In-situ characterization of well-arranged biomolecules on smart surfaces fabricated by scanning probe lithography |
| 초록 |
The surface immobilization of bio-macromolecules, such as proteins and cells, has long been a goal in biosensing, biomaterials, and tissue-engineering research and for use as detection systems, or as simplified model systems to study biointeractions. Patterning biomolecules at the nanometer scale gives a significant potential for studying how biological systems function at the macromolecular length scale. Herein, we combined the scanning probe lithography (SPL) with self-assembly method and/or wet etching method for the fabrication of positive and/or negative 3D micro/nano-structures. These prepared smart surfaces via SPL were then used to achieve site-selective arrays of biomolecules. In this study, we arrayed bio-molecules on the pre-fabricated silicon structures, even a single molecular level. In addition, direct observations of immobilized biomaterials were performed by real-time AFM imaging and tip-induced force-distance curve measuring. These studies provide understanding on characteristics and behaviors of biomolecules through visualization and manipulation at the single molecular level. |
| 저자 |
최인희1, 홍수린1, 이정진1, 이수승1, 양영인1, 김영훈2, 이종협1
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| 소속 |
1서울대, 2광운대 |
| 키워드 |
Atomic force microscopy; cell; scanning probe lithography; protein; surface
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| E-Mail |
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| 원문파일 |
초록 보기 |
