화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2018년 가을 (10/31 ~ 11/02, 제주국제컨벤션센터(ICC JEJU))
권호 22권 2호
발표분야 (특별세션) 에너지-화학 융복합 차세대기술의 현황 및 전망
제목 Bioelectrochemical conversion of CO2 to value added product formate using engineered CO2 reductase
초록 The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified as the key responsible enzyme for the conversion of carbon dioxide to formate in this study. Using a 2% methanol concentration for induction, microbial harboring the recombinant MeFDH1 expressing plasmid produced the highest concentration of formate in electrochemical reactor. 60 μM of sodium tungstate in the culture medium was optimal for the expression of recombinant MeFDH1 and production of formate. M. extorquens AM1 was successfully engineered by expressing MeFDH1 as recombinant enzyme to elevate the production of formate from CO2 after elucidating key responsible enzyme for the conversion of CO2 to formate.
저자 김용환
소속 UNIST
키워드 CO2 conversion; formate; formate dehydrogenase; Methylobacterium; cre-loxP knockout system; bioelectrochemical conversion
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