화학공학소재연구정보센터
학회 한국공업화학회
학술대회 2014년 가을 (11/12 ~ 11/14, 엑스코(대구))
권호 18권 2호
발표분야 나노-포스터
제목 Fluorescent Graphene Oxide-Peptide-Quencher Hybrids for Detection of Cell-Secreted Protease
초록 Graphene Oxide (GO) is an emerging luminescent nanomaterial with photo-stability and unique photoluminescence (PL) property. In addition, GO based sensing platform have attracted great attentions due to the featured properties of GO. Herein, we present a GO PL-based optical biosensor consisting of a luminescent GO donor covalently linked with a peptide-quencher complex for the simple, rapid and sensitive detection of proteases. Firstly, we examined the quenching efficiency of candidate quenchers for GO fluorescence, and investigated their quenching mechanism. The designed GO-peptide-quencher system was able to detect the specific proteases, chymotrypsin and matrix metalloproteinase-2 (MMP-2), by "turn-on" response of quenched GO fluorescence after proteolytic cleavage leading to release of quencher. The GO-peptide-quencher hybrid successfully monitored MMP-2 secreted from living cells with high sensitivity.
저자 양진경1, 곽선영2, 전수지2, 김혜인2, 강호만1, 경산1, 김종호2, 이윤식1
소속 1서울대, 2한양대
키워드 luminescent graphene oxide; optical protease sensor
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