| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Synthetic carbon flux redistribution for efficient production of cadaverine from galactose in Escherichia coli |
| 초록 |
Biological production of cadaverine has been suggested as an alternative route to replace petroleum-based polyamides process. Cadaverine should be produced with high yield and productivity from various sugars abundant in biomass. However, most microorganisms are not able to metabolize other biomass-derived sugars as fast as glucose. In this study, we engineered Escherichia coli to produce cadaverine with rapid assimilation of galactose, a promising future feedstock. To achieve this, genes related to the metabolic pathway were maximally expressed to amplify the flux toward cadaverine production via synthetic expression cassettes consisting of predictive and quantitative genetic parts (promoters, 5′-untranslated regions, and terminators). Furthermore, the feedback inhibition of metabolic enzymes and degradation/re-uptake pathways were inactivated to robustly produce cadaverine. |
| 저자 |
차상학1, 정규열1, 곽동훈1, 임현규1, 양진아1, 서상우2
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| 소속 |
1POSTECH, 2서울대 |
| 키워드 |
metabolic engineering |
| E-Mail |
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