| 학회 |
한국공업화학회 |
| 학술대회 |
2011년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터) |
| 권호 |
15권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
A hypersensitive CaMKII assay using [γ-32P]ATP and a PSPD-gold binding peptide fused substrate |
| 초록 |
Protein interactions in biological systems are frequently controlled by protein kinases. Among them, Ca2+/calmodulin-dependent protein kinase (CaMKII) has attracted much attention because aberrant protein kinase activity has been implication in a variety of human diseases.Using the pspd gene encoding the 32-kDa putative spermidine synthase (PSPD) of Selnomonas ruminantium, a PSPD-gold binding peptide fused substrate was cloned and purified to give a highly selective substrate for CaMKII.Using [γ-32P]ATP the substrate was used for the highly sensitive detection of a phosphorylation catalyzed by CaMKII. |
| 저자 |
최미희1, 고경철2, 박상현1
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| 소속 |
1한국원자력(연), 2한국생명공학(연) |
| 키워드 |
protein kinase assay; CaMKII assay; phosphorylation detection
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| E-Mail |
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