| 초록 |
In this study, stress hormone of cortisol has been detected by aptamer functionalized polymeric surface with sandwitched gold nanoparticle (Au NPs). Cortisol specific aptamer, which is 40-mer, was immobilized on the polymeric surface at first. Then, cortisol sample was applied to be bound to the aptamer. After that, cortisol monoclonal antibody (c-Mab) or aptamer conjugated 5 nm Au NPs were applied to evoke UV-Vis spectral absorption. Through the sandwiched binding of the Au NPs, quantitative analysis has been pursued to find a limit of detection (LOD) for the cortisol detection. |
