| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Development of cortisol detecting biosensor by aptamer based sandwitch assay |
| 초록 |
Cortisol, the representative stress hormone, can be detected in high sensitivity on glass and polymer surface with sandwiched assay method using aptamer and antibody functionalized gold nanoparticle (Au NPs). Cortisol specific aptamer, which is 40-mer, was immobilized on glass and polymer surface at first. Then, cortisol sample was applied to be specifically bound to the aptamer. After that, cortisol monoclonal antibody (c-Mab) or aptamer conjugated 5 nm Au NPs were applied to evoke UV-Vis spectral absorption. Through the sandwiched binding of Au NPs, quantitative analysis has been pursued to find a limit of detection (LOD) for the cortisol detection. |
| 저자 |
김길환, 김윤재, 양종민, 허혜연, 오승주, 신성철, 김초롱, Dhanashri Vitthal Desai, 이현호
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| 소속 |
Myongji Univ. |
| 키워드 |
biosensor; cortisol; sandwitch assay
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| E-Mail |
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