| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.446 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Polyethylene Gradient Surface with Biofunctional Groups |
| 초록 | A gradient surface is the surface on which a gradually varying chemical composition exists along its length. If bioactive molecule is introduced on a gradient surface, gradient bioactive molecule on gradient surface can illustrate various results through a single experiment. This can be a powerful tool to evaluate the ability of bioactive molecule. The aim of our research was to develop a simple applicable method using gradient surfaces for biomedical applications. Here, we describe the approach for design of gradient polyethylene (PE) surface prepared by an incorporation of biotin, followed by the binding of streptavidin derivatives on the biotinylated gradient surface Novel biotinylated gradient surface was designed to bind a streptavidin-FITC. The gradient polyethylene (PE) surface was prepared by a corona treatment with power increase along the PE length, and successively biotinylated gradient PE surface was prepared by the reaction of polyethylenimine (PEI) and biotin. Surface properties of the gradient PE surface changed according to PE length and chemical modification. The biotinylated gradient PE surface provided a continuous gradient of streptavidin-FITC through binding of avidin-biotin. The luminosity of fluorescences that correspond to a FITC of streptavidin on the biotinylated gradient PE surface increased gradually on the surface along the PE length. ACKNOWLEDGMENT. We would like to thank for funding from KMOICE (N11-A08-1402-05-1-3). |
| 저자 | 김문석1, 서광수1, 강길선2, 조선행1, 이해방1, 이태걸3, 문대원3 |
| 소속 | 1한국화학(연), 2전북대, 3표준과학(연) |
| 키워드 | Polyethylene; gradient; biomolecules |
