| 학회 |
한국화학공학회 |
| 학술대회 |
2021년 봄 (04/21 ~ 04/23, 부산 BEXCO) |
| 권호 |
27권 1호, p.508 |
| 발표분야 |
생물화공 |
| 제목 |
Enantiopure synthesis of (R)-gamma-valerolactone from levulinic acid by chemoenzymatic method |
| 초록 |
Cellulosic biomass is useful in industrial applications because of large-scale availability, low cost and non-food character. Utilization of cellulose, however, is hindered due to the production cost and catalytic power of cellulases. LA is a promising alternative, readily obtainable from cellulosic biomass, and used to synthesize value-added γ-valerolactone (GVL). GVL is a precursor to natural products, bioactive molecules, bio-fuels and carbon-based chemicals. This study has focused on four issues to make GVL synthesis efficient: high enantiopurity, high yield, usage of free form of LA as substrate rather than alkyl ester form, and moderate reaction conditions. A two-step chemoenzymatic strategy for asymmetric synthesis of (R)-GVL from LA was employed. In the first step, LA is converted into 4-hydroxyvaleric acid (4HV) by engineered 3-hydroxybutyrate dehydrogenase (e3HBDH) which was enhanced for catalytic activity on LA. In the second step, the lactonization of 4HV to (R)-GVL is promoted by dehydration with 1 % (v/v) sulfuric acid. The (R)-GVL was successfully produced with nearly 100% yield and high enantiopurity (> 99 % ee) from the free acid form of levulinic acid. |
| 저자 |
오규림1, 김유림2, 정지영1, 이완서3, 이도훈4, 연영주5
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| 소속 |
1Gangneung-Wonju National Univ., 2Department of Biochemical Engineering, 3Gangneung, 425457, 5Korea |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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