| 학회 | 한국화학공학회 |
| 학술대회 | 2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 | 28권 1호, p.841 |
| 발표분야 | [주제 4] 탄소중립(CCUS) |
| 제목 | Component interactions for the electron transfer from reductase to hydroxylase |
| 초록 | Methane has a high heat capacity than CO2, and its impact is critical to global warming. Soluble methane monooxygenase (sMMO) converts methane to methanol under ambient conditions. sMMO oxidizes methane to methanol using at least three proteins, including the regulatory protein (MMOB) and reductase (MMOR). The MMOH-MMOB complex provides details about the hydroxylation reactions that occur inside MMOH by MMOB’s allosteric effects. This study, investigated the structure of MMOR and analyzed the first X-ray structure of MMOR FAD-binding domain to understand the electron transfer routes. The six β-strands with two α-helices generated a β-barrel. The sidechains of hydrophobic amino acids are oriented toward the inner space, and the sidechains of hydrophilic amino acids are oriented toward the solvent-accessible area. We realized that four hydrogen bonds to the FAD cofactor could be the electron transfer pathways, and mutational studies identified that Tyr160 is a key residue for its catalytic cycle in sMMO. |
| 저자 | 이채민1, 이승재2 |
| 소속 | 1Department of Chemistry, 2Jeonbuk National Univ. |
| 키워드 | 에너지 환경(Energy and Environment) |
| 원문파일 | 초록 보기 |
