| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 가을 (10/06 ~ 10/08, 제주 ICC) |
| 권호 |
39권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Selective stimulation of caveolae-mediated endocytosis and hepatocyte-targeting by an osmotic polylactitol-based gene transporter |
| 초록 |
Here, a new multifunctional polymeric nanocarrier, polylactitol-based gene transporter (PLT) is developed by crosslinking low molecular weight branched polyethylenimine (b-PEI) with lactitol diacrylate. Polylactitol has multifunctional properties having three key functions for successful gene delivery: 1) hyperosmotic part trigger caveolae-mediated endocytosis, 2) galactose part can deliver genes into the hepatocyte via receptor-mediated endocytosis, and 3) PEI part assists rapid endosomal escape of gene due to proton sponge effect. As expected, PLT/DNA nanocomplexes showed low cytotoxicity, significant transfection efficiency, hepatocyte-specific targeting, and transition of cellular uptake pathway into caveolae-mediated endocytosis avoiding lysosomal degradation. Our results suggested that polylactitol-based gene transporter (PLT) is safe and efficacious vector, which will highlight its potential in the treatment of hepatic diseases. |
| 저자 |
김영동, 박태은, 조계수, 최윤재, Rohidas B. Arote, 조종수
|
| 소속 |
서울대 |
| 키워드 |
Gene delivery; polylactitol; PEI; hepatocyte; caveolae-mediated endocytosis
|
| E-Mail |
|
