| 초록 |
The comparison of structures and sequences of homologous proteins from thermophilic and mesopjilic organisms could provide the important clues to stabilize proteins. We developed a protein residual pattern to find out the structural differences between stable proteins and less-stable proteins. The method is based on both of the conventional methods, the comparative study and systematic analysis. It is distinguished form other previous works in that it is a residue-based analysis, that is, it enables to analyze the structural characteristics of model protein group in the residual view. The method categorizes the residues of proteins into eight classes under their own structure index, and could provide a residual pattern of structural properties in protein group. 20 pairs set of thermophilic and mesophilic proteins were analyzed to find out the residual differences of thermophilic proteins, which would be the residual factors applied to develop a strategy for stabilizing proteins. |
