| 초록 |
In this study, we prepared visible light-cured glycol chitosan (GC) hydrogels as biomaterial scaffolds for skin tissue regeneration. As a functional group for photo-curing, glycidyl methacrylate (GM) was conjugated to GC through epoxy ring opening reaction, confirmed by 1H NMR analysis. In order to improve skin tissue regeneration, we prepared several visible light-cured GC hydrogel systems containing biomolecules such as vascular endothelial growth factor (VEGF), platelet-derived growth factor-BB (PDGF-BB), curcumin (CUR) and inclusion complex between beta-cyclodextrin (β-CD) and CUR. Storage modulus of the hydrogel systems after visible light irradiation of hydrogel precursor solutions was larger than loss modulus, demonstrating formation of hydrogel by three-dimensional crosslinking. In addition, the hydrogel systems showed interconnected porous structures, observed by SEM. The porosity affected the release behavior of biomolecules, resulting in controlled release in a sustained manner along with sharp initial burst. In vivo studies showed the biomolecule-loaded GC hydrogel systems further enhanced skin tissue regeneration compared to GC itself. This result indicated that GC hydrogel systems covered to wound site not only prevents impurities outside, but also serves as a reservoir of biomolecules that lead to wound healing acceleration, promoting skin tissue regeneration. Therefore, visible light-cured GC hydrogel systems containing biomolecules may have potential as wound dressings for clinical use. Acknowledgments: This research was supported by grants (Grant no. 10052595 and 10047811) of the Ministry of Trade Industry and Energy (MOTIE, Korea), grants (2014R1A1A1002697 and 2017R1D1A1B03033195) of National Research Foundation (NRF, Korea). |
