| 초록 |
The hemagglutinin of influenza virus binds to sialicacid, which is a main residue of host cell receptor, to initiatevirus entry. Therefore, we have studied the effect of sialyl ligands conjugatedto nano-scaffolds on the binding of hemagglutinin has been analyzed to developinhibitors to block influenza virus cell entry. In this study, we developeda range of synthetic multivalent sialyl ligands with controlled-valencies, interspacingbetween sialyl motifs and net charge. Sialyl groups were coupled tolysine-based dendritic molecules via various lengths and charges of flexiblelinkers, which were confirmedby 1H-NMR and mass spectroscopy. Next, the binding affinityand inhibition efficiency of the receptors were investigated against influenzaA and B, using surface plasmon resonance and plaque inhibitionassays. Based on these results,we investigated the structure-function relationships of sialylligands. |
