| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 봄 (04/23 ~ 04/25, 제주ICC) |
| 권호 |
14권 1호, p.515 |
| 발표분야 |
생물화공 |
| 제목 |
ssDNA aptamer-based SPR biosensor for early diagnosis of type 2 diabetes by RBP4 detection |
| 초록 |
Retinol-binding protein 4 (RBP4) is one of the adipokines which contributes to insulin resistance under the condition of obesity and type 2 diabetes. The level of RBP4 increases in insulin resistance state. We have developed an aptamer-based SPR biosensor for sensing RBP4 in serum. An ssDNA aptamer with high affinity and specificity to bind RBP4 was selected from a random pool of 3x1016 molecules by SELEX method. The RBP4 aptamer obtained for the first time was immobilized on a gold chip and used for a label free detection of RBP4 by Surface Plasmon Resonance (SPR). The dissociation constant of the aptamer was calculated to be 0.2±0.03 μM by SPR response units, and the minimum detectable concentration of this aptamer-based SPR biosensing system was 75 nM. Analysis of RBP4 in artificial serum using SPR was compared with ELISA and western-blot analysis. Our results indicated that the RBP4-specific aptamer-based SPR biosensor gave better dose-dependent responses and was more sensitive than ELISA assays. As such, this RBP4 aptamer-based SPR biosensor can be used to monitor the RBP4 levels within the serum as an indicator of type 2 diabetes. |
| 저자 |
이수진, Javed H. Niazi, 김연석, 구만복
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| 소속 |
고려대 |
| 키워드 |
ssDNA aptamer; RBP4; SPR
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| E-Mail |
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| 원문파일 |
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