| 초록 |
Hydrogels have offered various biological applications such as scaffold, wearable or implantable devices and bio-sensors due to their advantages including (i) high water content and easy functional modification enabling biological reactions taken inside the gel, (ii) high stability and biocompatibility, and (iii) prevention of non-specific adsorptions of proteins. Inflammatory enzymes, such as matrix-metalloproteinase (MMP) 2&9 (Gelatinase A&B), have been investigated widely because of their roles in development of inflammation and/or tumor metastasis. Specifically, during degradation of extracellular matrix by MMP-2&9, cleavage of specific peptide sequences is accompanied. Herein, we developed MMP-9 activatable intraocular lens-shaped fluorogenic hydrogel sensor using MMP-9 targetable peptide probe. In detail, vinyl group terminated peptide probe was conjugated during poly(ethylene diacrylamide) (PEGDAAm) hydrogel polymerization. PEGDAAm hydrogel showed improved stability to acidic or basic conditions. Peptide probe inside the hydrogel sensor was cleaved with existence of MMP-9, becoming fluorescence off-state into on-state by detachment of dye and quencher. Fluorescence intensity was increased with concentration of MMP-9 increased. |
