| 학회 |
한국화학공학회 |
| 학술대회 |
2006년 가을 (10/27 ~ 10/28, 고려대학교) |
| 권호 |
12권 2호, p.1834 |
| 발표분야 |
생물화공 |
| 제목 |
Extraction and Purification of Alkali-soluble glucan Produced from Saccharomyces cerevisiae JUL3 Using Molasses and Corn steep Liquor and Measurement of Anti-complementary Activity |
| 초록 |
Molasses and cone steep liquor have been used extensively as a medium source for commercial production of various bioproducts. These media were used as cheaper carbon and nitrogen sources for alkali-soluble glucan production by Saccharomyces cerevisiae JUL3. Glucans are usually grouped into several fractions. The alkali-soluble β-glucan fraction consists of a β-(1,3)-linked backbone containing 3% β-(1,6)-linkages, and is responsible for structural integrity of the cell wall. These compounds show a variety of biological activities by stimulation of the immune system. For the production of alkali-soluble glucan, the optimization of culture media (molasses and corn steep liquor) was performed by response surface methodology (RSM). The cell wall fraction was prepared by alkali extraction. The alkali-soluble glucan was purified by DEAE ion-exchange chromatography, Concanavalin-A chromatography, and freeze-drying. Finally, we tested anti-complementary activity of the alkali-soluble glucan. |
| 저자 |
김영환1, 강성우1, 김동오1, 장효일1, 윤철원1, 백현동2, 강창원2, 김승욱1
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| 소속 |
1고려대, 2건국대 |
| 키워드 |
Alkali-soluble glucan; Saccharomyces cerevisiae JUL3; Molasses; Corn steep liquor; Anti-complementary activity
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| E-Mail |
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| 원문파일 |
초록 보기 |
