| 학회 |
한국화학공학회 |
| 학술대회 |
2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 |
28권 1호, p.1251 |
| 발표분야 |
[주제 12] 화학공학일반(부문위원회 발표) |
| 제목 |
Efficient production of β-alanine using metabolically engineered Corynebacterium glutamicum |
| 초록 |
β-Alanine is an important beta-amino acid with growing demand in the fine chemical and pharmaceutical industries. The current industrial production of β-alanine still relies on chemical synthesis, with harmful raw materials and harsh operational conditions, causing the final product difficult to meet safety requirements for human use. Thus, alternative routes of β-alanine production are highly desirable to enhance environmental sustainability. In this study, we constructed a Corynebacterium glutamicum for high-level β-alanine production through systematic experimental design and application of genome-scale metabolic simulation-based strategies, including screening the best enzymes for β-alanine biosynthesis and enhancement of glucose uptake system and anaplerotic pathway. Our integrated metabolic engineering strategies generated a strain that could produce 166.6 g/L of β-alanine with 1.74 g/L/h productivity from fed-batch fermentation, the highest titer and productivity ever reported. This work presents a promising result for high-titer production of β-alanine using recombinant C. glutamicum achieved by systems metabolic engineering strategies. |
| 저자 |
Ghiffary Mohammad Rifqi, Joshua Julio Adidjaja, 김현욱
|
| 소속 |
KAIST |
| 키워드 |
생물화공(Biochemical Engineering) |
| E-Mail |
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| 원문파일 |
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