| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 가을 (10/11 ~ 10/12, 창원컨벤션센터) |
| 권호 |
38권 2호 |
| 발표분야 |
대학원생 구두발표(발표10분) |
| 제목 |
Transfection and intracellular trafficking properties of carbon dot-gold nanoparticle molecular assembly conjugated with PEI-pDNA |
| 초록 |
To understand the mechanisms of polymeric gene delivery, monitoring of polyplex dissociation inside cell is one of the most important issues. Even though there are many reports about cellular uptake and endosomal escape, the study on the dissociation of polyplex has not been reported thoroughly. Herein, we developed a delivery system which can monitor the dissociation of polyplex, and deliver the pDNA effectively. The polyplex dissociation was monitored using carbon dot (CD) since its excellent optical properties and biocompatibility. The fluorescence of CD is quenched by Au nanoparticle when they interact with non-labeled pDNA since CD and Au surface is modified with highly cationic polymer, polyethylenimine (PEI). When the pDNA was dissociated, the fluorescence of CD recovered and this changes enabled the monitoring of dissociation. In addition, this system showed high transfection efficiency with high cell viability which is the prerequisite of the effective carrier. |
| 저자 |
김진환, 김현우, Kaushik Singha, 김원종
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| 소속 |
포항공과대 |
| 키워드 |
Carbon dot; gold nanoparticle; polyethylenimine; gene delivery; carrier-DNA dissociation
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| E-Mail |
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