| 초록 |
Klebsiella oxytoca can convert various carbon sources to 2,3-butanediol (2,3-BD), especially (2R,3S)-BD. To reduce the formation of byproducts, K. oxytoca strain was engineered by deleting the ldhA and pflB genes based on in silico analysis. Then, we optimized the fermentation conditions and produced 113 g/L of (2R,3S) rich-BD (0.45 g/g, 2.1 g/L/h). Additionally, we carried out a number of tests for (2R,3S) rich-BD produced from the K. oxytoca strain to evaluate the 2,3-BD application as a cosmetic material. [This work was supported by the Biochemical Industry Promoting Technology Development Project (No. 10050407) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).] |
