| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 봄 (04/14 ~ 04/15, 전경련회관) |
| 권호 | 30권 1호, p.281 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Biological evaluation of mouse NIH/3T3 fibroblast cells onto different wettability |
| 초록 | The adhesion and proliferation of mammalian cells on polymeric biomaterials depend on the surface characteristics such as wettability, chemistry, charge and roughness. In order to recognize the correlation between the adhesion and proliferation of mouse NIH/3T3 fibroblasts and surface property, radio frequency generated plasma treatment on low density polyethylene (LDPE) has been carried out.1 Plasma surface treatment is regarded as a unigue polymer surface chemical modification technique. It can be used for the chemical modification of polymer surfaces without affecting their bulk properties.2 The modified LDPE surfaces were characterized by measuring the static water contact angle. The adhesion and proliferation of cells on LDPE films were characterized by cell counting, reverse transcription-polymerase chain reaction (RT-PCR) and SEM. The water contact angle of the film surface decreased with plasma treatment time. Proto-oncogenes (c-myc, c-fos) and tumor suppressor gene (p53) showed maximum expression with contact angle of 60 ~ 70 ° range of LDPE film. By cell counting, we confirmed that the rate of cell proliferation appeared the higher on the film surface of the contact angle of 60 ~ 70 °. We concluded that the surface wettability is an important role for the growth of NIH/3T3 cells. Figure 1. Agarose gel electrophoresis ① β-actin 1 day 2 day 3 day ② p53 1 day 2 day 3 day Acknowledgement This work was supported by KMOHW (0405-BO01-0204-0006). Reference 1. E. J. Kim, C. S. Park, and M. S. Kim, polymer, 28, 218 (2004). 2. G. Akovali, Z. M. O. Rzaev and D. G. Mamedov, Eur. Polym. j. 32, 375 (1996). |
| 저자 | 김순희1, 박기숙1, 홍금덕1, 김선경1, 김문석2, 이해방2, 이종문1, 강길선1 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | plasma; PE film; RT-PCR |
