| 초록 |
Horseradish peroxidase (HRP) and hydrogen peroxide (H2O2)-catalyzed hydrogelation system has attracted much attention due to their ease of handling and controllable gel properties. In this study, we demonstrated that the hydrogelation can be achieved using calcium peroxide (CaO2) as an H2O2-generating reagent to gradually supply radical source for HRP-catalyzed crosslinking reaction. The physico-chemical properties of hydrogels can be controlled by varying concentrations of HRP and CaO2. In addition, hydrogels exhibited killing efficacy against both gram-negative and gram-positive bacteria, dependent on the H2O2 and Ca2+ release amount. Furthermore, the cellular behaviors (adhesion, spreading and proliferation) in 2D and 3D microenvironments showed excellent biocompatibility of hydrogels. This work suggested the great potential of hydrogels formed by HRP/CaO2 for a wide range of biomedical applications. |
