| 초록 |
We developed unique and powerful fluoroimmunosensor named Quenchbody that works on the novel principle of antigen-dependent removal of quenching effect on fluorophores. The outstanding advantage of Quenchbody assay is its simplicity, which can be carried out by just mixing the Quenchbody with antigen and measuring its fluorescence, while almost all the traditional immunoassays require several incubation/washing steps. Using this convenient method, we have successfully quantified various target antigens including phosphorylated serine residues in vimentin, narcotics such as morphine, influenza hemagglutinin, and hen egg lysozyme. Moreover, using this Quenchbody, cellular imaging of osteocalcin produced by differentiated osteoblast cells or claudin proteins on tumor cells was successfully accomplished. |
