| 학회 | 한국화학공학회 |
| 학술대회 | 2013년 가을 (10/23 ~ 10/25, 대구 EXCO) |
| 권호 | 19권 2호, p.1499 |
| 발표분야 | 생물화공 |
| 제목 | Rapid and precise identification of foodborne pathogens using antibody conjugated magnetic nanoparticles coupled with MLPA-CE-SSCP |
| 초록 | Foodborne illness is a serious problem in public health and food industries. The conventional culture method is known as the most reliable technique, however, it takes 2~3 days. Therefore, recently many researchers have focused towards the rapid detection methods. Here, we developed a rapid pathogen detection system using antibody conjugated magnetic nanoparticles (Ab-MNPs) coupled with stuffer-free MLPA-CE-SSCP. The pathogens were concentrated by Ab-MNPs and analyzed with MLPA-CE-SSCP. As a result, the 1 cfu/ml of S. enteritidis were successfully detected in 100 ml of samples within 10 hours. These results indicate that Ab-MNP with MLPA-CE-SSCP system has considerable potential in clinical diagnosis and food safety. This work was supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government(MSIP) (No. 2012R1A2A2A01009868). |
| 저자 | 정보람1, 신기원2, 권동훈3, 최 웅1, 전상민3, 정규열4 |
| 소속 | 1POSTECH 시스템생명공학부, 2POSTECH 환경에너지(연), 3POSTECH 화학공학과, 4POSTECH 화학공학과 / POSTECH 시스템생명공학부 |
| 키워드 | |
| 원문파일 | 초록 보기 |
